The Catalytic Performance of Penicillin G Acylase in Different Organic Solvents

Dong-Lei LIU, Zhen-Bin CHEN, Zhen LIU


This work investigated the catalytic performance of PGA engineered with a variety
of functional groups in organic solvents at room temperature for different interval, such as nalkanes, chlorinated methane, alcohols and acids. The major research highlights are listed as: ① With the extension of time, as for hydrophobic organic solvent, catalytic activity retention rate (Cr) decreased and achieved equilibrium gradually, in a series of solvent, the smaller log P, the lower Cr; as for hydrophilic organic solvent, Cr increased first and then decreased to an equilibrium value, in a series of solvent, the smaller log P, the higher Cr. ②With the increasing of the solvent molecular size (l), equilibrium Cr achieved showed different trends: when l < 4.4 Å, equilibrium Cr achieved was fast and not affected by l; when 4.4 Å < l < 6.9 Å, it was slow and increased with the increasing of l, when l > 6.9 Å, it was fast and decreased with the increasing of l. ③ In the series solvents, PGA treated with polyalcohol, especially glycerine, its Cr reached maximum and maintained stability.


Catalytic activity retention rate, Equilibrium time, Organic solvent, PGA conformation


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