Inducing Adventitious Buds Regeneration from Leaf Explants of Purple Coneflower (Echinacea Purpurea L.)

Bing-zhen LIU, Hong-jie LIU, Ting-ting XIE, Kai-zhe LIU, Xiao-peng LI, Guang-jian LIU, Ying-bin XUE, Miao CHEN, Ying LIU, Shao-xia YANG

Abstract


In the present study, purple coneflower leaves were used as explants to explore the effects of basic medium, CuSO4, and Diethyl aminoethyl hexanoate (DA-6) on induction of adventitious buds. The results shown that MS medium was the most suitable basic medium for inducing adventitious buds regeneration from leaf explants. When the concentration of CuSO4 was at 0.5 mg/L, a great effect of induction of adventitious buds was obtained (90%). Meanwhile, the optimal culture condition for the proliferation of adventitious buds was MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA).

Keywords


Echinacea Purpurea, Leaf, Adventitious Buds, Regeneration


DOI
10.12783/dteees/icepe2019/28936

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