Active Biostimulation of Environmental Soils Contaminated with Petroleum Lubricants

Jiang WU, Xian-ming ZHANG, Yu-ming HUANG, Ping LIU, Bo-shui CHEN


A new idea, active biostimulation, was put forward that direct addition of microbial nutrients into unreadily biodegradable petroleum-based lubricants is possible to stimulate biodegradation of the lubricants when they enter the environment, thus to help in situ “active” bioremediation of lubricant-contaminated areas. In the present study, small amount of nutritious amino acids, viz. oleoyl glycine, lauroyl alanine and lauroyl glycine, as “active biostimulators” were doped into a petroleum lubricating oil, respectively. The biodegradation capacities of neat oil and the amino acid-doped oils in environmental soils under specific conditions were determined and their biodegradation kinetics simulated based on the exponential kinetics model. The results demonstrated that oleoyl glycine, lauroyl alanine and lauroyl glycine were efficient in boosting biodegradation of lubricating oils in soils. Furthermore, biodegradation of lubricating oils in soils were well modeled by the first-order exponential kinetics. The biodegradation kinetics for the amino acid-doped oils in soils clearly displayed greater biodegradation reaction rates and thereby shorter biodegradation half-lives.


Lubricant, Biodegradation, Biostimulation, Kinetics, Amino acid, Soil


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